9. Gene Set Enrichment Analysis (GSEA)
Computational method to determine if a set of genes shows statistically significant differences in control and Tau over expressing conditions.
::: {.left} - Plot genes
::: {style=“width: 45%; text-align: left;”} 
::: {.right} - Plot pathways
::: {style=“width: 45%; text-align: right;”}
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10. Discussion based on the GSEA/conclusion
Gamma and Alpha response- cytokine, regulation of immune response, trigger JAK-STAT3 pathway. JAK-STAT3 pathway- immunity, cell division, and other cellular processes.
–Promote neuroinflammation in neurodegenerative disorders like Alzheimer’s by initiation innate immunity, adaptive immune mechanisms, and neuroinflammatory response. (https://pubmed.ncbi.nlm.nih.gov/36614305/#:~:text=The%20JAK%2FSTAT%20signaling%20pathway%20is%20one%20of%20the%20critical,and%20finally%2C%20constraining%20neuroinflammatory%20response.)
-Supports: link of Tau overexpression to neurodegenerative disorders like Alzheimer’s.
Challenges
Data handling and cleaning: setting arbitrary boundaries without losing too much data or leaving in unnecessary data.